Vectors for Dual Expression of Target Genes in Bacterial and Mammalian Cells

Vectors for dual expression of target genes in bacterial and mammalian cells.

For protein production and purification, many different cell systems ranging from bacteria to mammalian cell lines are commonly used. High-level expression of recombinant proteins in E. coli is often achieved using the strong yet tightly regulatable T7 promoter system (23). Many convenient T7 promoter-based bacterial expression vectors are commercially available from different sources and are w...

Dual-expression vectors for efficient protein expression in both E. coli and mammalian cells.

In silico cloning and bioinformatics study of Brucella melitensis Omp31 antigen in different mammalian expression vectors

Brucella melitensis, as a pathogenic gram-negative intracellular bacterium, causes brucellosis in animals and humans. According to literature, the B. melitensis outer membrane protein 31 (Omp31) is considered as an important vaccine candidate against brucellosis. The aim of the current study was to compare three different expression constructs containing B. melitensis Omp31 antigen using bioinf...

Construction of Plant Expression Vectors Harboring WRI1 and LPAAT Genes and Its Transformation in Tobacco Plants

In oilseed crops, a number of genes involved in the production of triacylglycerol have been identified that changes in their expression have increase the seed oil content. WRI1 and LPAAT are key genes in this synthetic pathway that their overexpression can increase the oil content. In this study, the expression vectors carrying WRI1 and LPAAT genes were designed and constructed for genetic tran...

Recombinant baculoviruses as expression vectors for insect and mammalian cells.

Baculovirus expression vectors are widely used for expressing heterologous proteins in cultured insect cells. Recent advances include further development of the system for production of multi-subunit protein complexes, co-expression of protein-modifying enzymes to improve heterologous protein production, and additional applications of baculovirus display technology. The application of modified ...